ZIKV (strain Zika SPH2015) NS5 Protein, His Tag
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产品编号
KMP4929
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别名
寨卡病毒非结构蛋白5, ZIKV NS5, Nonstructural Protein 5, ZIKV NS5 Protein
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规格
- 50ug
- 100ug
- 200ug
产品介绍
| Alias | 寨卡病毒非结构蛋白5, ZIKV NS5, Nonstructural Protein 5, ZIKV NS5 Protein |
| Catalog Number | KMP4929 |
| Product Description | The ZIKV (strain Zika SPH2015) NS5 Protein(KMP4929) is produced in Insect Cells. A DNA sequence encoding the Zika virus (ZIKV) (strain ZikaSPH2015) NS5 (ALU33341.1) (Lys2772-Leu3423) was expressed with a polyhistidine tag at the C-terminus. |
| Molecular Name | ZIKV NS5 |
| Species | ZIKV |
| Host | Insect Cells |
| Size | 50ug, 100ug, 200ug |
| Purity | >95% as determined by SDS-PAGE |
| Purification | Affinity purification |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Formulation | PBS, pH7.4 |
| Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
FAQ
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Q1-NS5蛋白的功能是什么？
NS5是病毒RNA依赖的RNA聚合酶（RdRp），负责基因组复制，并具有甲基转移酶活性，是抗病毒药物（如核苷类似物）的靶点。
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Q2-如何检测NS5的酶活性？
使用荧光底物（如NTP类似物）或体外RNA合成实验，通过凝胶电泳检测产物。
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Q3-能否用于高通量药物筛选？
是的，适用于96/384孔板形式的酶活抑制实验，筛选RdRp或甲基转移酶抑制剂。
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Q4-His标签是否影响蛋白功能？
标签位于C端，经活性验证不影响催化功能或底物结合。
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Q5-储存稳定性如何？
添加10%甘油和1 mM DTT，-80℃保存可维持活性6个月以上。
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