MERS-CoV Spike Protein(ECD), His Tag
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产品编号
KMP4882
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别名
中东呼吸综合症冠状病毒刺突蛋白, MERS-CoV Spike, S Protein, MERS-CoV Spike Protein
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规格
- 50ug
- 100ug
- 200ug
产品介绍
| Alias | 中东呼吸综合症冠状病毒刺突蛋白, MERS-CoV Spike, S Protein, MERS-CoV Spike Protein |
| Catalog Number | KMP4882 |
| Product Description | The MERS-CoV Spike Protein(KMP4882) is produced in Insect Cells. A DNA sequence encoding the MERS-CoV extracellular domain of spike protein (AFS88936.1) (Met1-Trp1297) was fused with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro. |
| Molecular Name | MERS-CoV Spike |
| Species | MERS-CoV |
| Host | Insect Cells |
| Size | 50ug, 100ug, 200ug |
| Purity | >90% as determined by SDS-PAGE |
| Purification | Affinity purification |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Formulation | 20mM Tris, 500mM NaCl, 10% Glycerol, pH7.5 |
| Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
FAQ
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Q1-MERS Spike蛋白的宿主受体是什么？
结合宿主DPP4（二肽基肽酶4），介导病毒入侵呼吸道和肠道上皮细胞。
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Q2-该蛋白能否用于治疗性抗体制备？
是的，如单抗REGN3048/3051靶向Spike RBD，可通过中和实验筛选类似抗体。
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Q3-如何验证三聚体稳定性？
动态光散射（DLS）检测粒径均一性，高温（37℃）孵育后SEC验证无降解。
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Q4-是否支持假病毒包装？
是的，与慢病毒载体共转染可生成MERS假病毒，用于中和实验（需BSL-2条件）。
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Q5-糖基化修饰是否影响抗体识别？
哺乳动物细胞表达保留天然糖基化，去糖基化（PNGase F处理）可能暴露隐藏表位。
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