H3N1 (A/swine/England/704563/1995) HA Protein, His Tag
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产品编号
KMP4691
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别名
甲型流感病毒H3N1血凝素蛋白, H3N1 HA, Hemagglutinin Protein, H3N1 HA Protein
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规格
- 50ug
- 100ug
- 200ug
产品介绍
| Alias | 甲型流感病毒H3N1血凝素蛋白, H3N1 HA, Hemagglutinin Protein, H3N1 HA Protein |
| Catalog Number | KMP4691 |
| Product Description | The H3N1 (A/swine/England/704563/1995) HA Protein(KMP4691) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/swine/England/704563/1995 (H3N1)) hemagglutinin (AFR76416) (Met1-Ser525), termed as HA, was expressed with a polyhistidine tag at the C-terminus. |
| Molecular Name | H3N1 HA |
| Species | H3N1 |
| Host | HEK293 Cells |
| Size | 50ug, 100ug, 200ug |
| Purity | >95% as determined by SDS-PAGE |
| Purification | Affinity purification |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Formulation | PBS, pH7.4 |
| Background | The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity. |
| Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
FAQ
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Q1-H3N1亚型HA蛋白的独特功能是什么？
H3亚型HA通常具有广泛的受体结合能力，可能促进病毒在猪群中的适应性传播，研究其结构可揭示宿主特异性机制。
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Q2-该毒株在英国猪群中的流行病学意义如何？
1995年分离的毒株反映了H3N1在欧洲猪群中的早期传播，为流感病毒进化史研究提供重要数据。
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Q3-该蛋白的表达系统是什么？
采用杆状病毒-昆虫细胞（Sf9）表达系统，确保蛋白正确折叠及糖基化修饰。
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Q4-能否用于多价疫苗开发？
是的，与其他亚型HA蛋白联用，可设计针对猪流感的广谱保护性疫苗。
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Q5-蛋白的纯度如何保证？
通过镍柱亲和层析和分子筛纯化，SDS-PAGE验证纯度≥95%。
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