H1N2 (A/swine/Tainan/46-4/2005) HA Protein, His Tag
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产品编号
KMP4676
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别名
甲型流感病毒H1N2血凝素蛋白, H1N2 HA, Hemagglutinin Protein, H1N2 HA Protein
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规格
- 50ug
- 100ug
- 200ug
产品介绍
| Alias | 甲型流感病毒H1N2血凝素蛋白, H1N2 HA, Hemagglutinin Protein, H1N2 HA Protein |
| Catalog Number | KMP4676 |
| Product Description | The H1N2 (A/swine/Tainan/46-4/2005) HA Protein(KMP4676) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/swine/Tainan/46-4/2005(H1N2)) hemagglutinin (ACO07051.1)(Met1-Ser524), termed as HA, was expressed with a polyhistidine tag at the C-terminus. |
| Molecular Name | H1N2 HA |
| Species | H1N2 |
| Host | HEK293 Cells |
| Size | 50ug, 100ug, 200ug |
| Purity | >95% as determined by SDS-PAGE |
| Purification | Affinity purification |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Formulation | PBS, pH7.4 |
| Background | The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity. |
| Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
FAQ
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Q1-该HA蛋白在猪流感病毒中的作用是什么？
HA（血凝素）蛋白介导病毒与宿主细胞受体的结合，并促进膜融合，是猪流感病毒入侵宿主的关键蛋白，也是疫苗研发的主要靶点。
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Q2-台湾猪源H1N2毒株的研究意义是什么？
猪是流感病毒重组的重要宿主，研究该毒株HA蛋白有助于追踪H1N2在猪群中的进化及潜在跨物种传播风险。
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Q3-His标签是否会影响HA蛋白的免疫原性？
His标签位于C末端，经ELISA和中和实验验证，不影响抗原表位识别及抗体结合活性。
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Q4-该蛋白适用于哪些应用场景？
可用于猪流感血清学诊断、疫苗抗原设计及跨物种传播机制研究。
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Q5-如何验证HA蛋白的受体结合活性？
通过红细胞凝集实验（HA Assay）或表面等离子共振（SPR）检测其与唾液酸受体的结合能力。
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