H8N4 (A/pintail duck/Alberta/114/1979) HA Protein(ECD), His Tag
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产品编号
KMP4495
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别名
甲型流感病毒H8N4血凝素蛋白, H8N4 HA, Hemagglutinin Protein, H8N4 HA Protein
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规格
- 50ug
- 100ug
- 200ug
产品介绍
| Alias | 甲型流感病毒H8N4血凝素蛋白, H8N4 HA, Hemagglutinin Protein, H8N4 HA Protein |
| Catalog Number | KMP4495 |
| Product Description | The H8N4 (A/pintail duck/Alberta/114/1979) HA Protein(KMP4495) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus (A/pintail duck/Alberta/114/1979(H8N4)) hemagglutinin (ABB87729.1) (Met1-Gln529), termed as HA, was expressed with a polyhistidine tag at the C-terminus. |
| Molecular Name | H8N4 HA |
| Species | H8N4 |
| Host | Insect Cells |
| Size | 50ug, 100ug, 200ug |
| Purity | >90% as determined by SDS-PAGE |
| Purification | Affinity purification |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Formulation | PBS, pH7.4 |
| Background | Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of the influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme that facilitates virus release and efficient spread of the progeny virus from cell to cell. Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. The monoclonal or polyclonal antibody can be raised with protein based antigen or peptide-based antigen. Antibodies raised with protein-based antigen could have better specificity and/or binding affinity than antibodies raised with peptide based antigen, but the cost associated with the recombinant protein antigen is usually higher. Anti-influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection. Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies. |
| Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
FAQ
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Q1-HA胞外域（ECD）是否包含完整的受体结合域（RBD）？
是的，包含完整的RBD，用于研究病毒与宿主唾液酸受体的相互作用。
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Q2-能否用于跨物种传播机制研究？
可以，结合哺乳动物细胞模型，分析HA的受体结合偏好性（如α-2,3 vs. α-2,6连接）。
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Q3-如何验证蛋白的正确折叠？
通过ELISA检测其与广谱中和抗体（如CR6261）的结合能力，确保构象正确。
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Q4-是否支持冻干粉和液体两种形式？
支持，冻干粉可常温运输，液体需-80℃保存，用户可按需选择。
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Q5-是否提供定制突变体（如增强稳定性）？
支持，可引入Cys突变或二硫键优化，增强蛋白热稳定性。
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400-621-6806 -
614353794 -
周一至周五 9:00-18:00 -
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