H11N2 (A/duck/Yangzhou/906/2002) HA Protein, His Tag
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产品编号
KMP4487
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别名
甲型流感病毒H11N2血凝素蛋白, H11N2 HA, Hemagglutinin Protein, H11N2 HA Protein
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规格
- 50ug
- 100ug
- 200ug
产品介绍
| Alias | 甲型流感病毒H11N2血凝素蛋白, H11N2 HA, Hemagglutinin Protein, H11N2 HA Protein |
| Catalog Number | KMP4487 |
| Product Description | The H11N2 (A/duck/Yangzhou/906/2002) HA Protein(KMP4487) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus (A/duck/Yangzhou/906/2002(H11N2)) hemagglutinin (AAY85533.1) (Met1-Lys528), termed as HA, was expressed with a polyhistidine tag at the C-terminus. |
| Molecular Name | H11N2 HA |
| Species | H11N2 |
| Host | Insect Cells |
| Size | 50ug, 100ug, 200ug |
| Purity | >90% as determined by SDS-PAGE |
| Purification | Affinity purification |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Formulation | PBS, pH7.4 |
| Background | Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of the influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme that facilitates virus release and efficient spread of the progeny virus from cell to cell. Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. The monoclonal or polyclonal antibody can be raised with protein based antigen or peptide-based antigen. Antibodies raised with protein-based antigen could have better specificity and/or binding affinity than antibodies raised with peptide based antigen, but the cost associated with the recombinant protein antigen is usually higher. Anti-influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection. Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies. |
| Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
FAQ
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Q1-该HA蛋白是否适用于家禽疫苗开发？
是的，作为灭活疫苗或亚单位疫苗的候选抗原，评估其对鸭群的免疫保护效果。
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Q2-如何保证蛋白的高纯度？
采用两步纯化（His标签亲和层析+离子交换层析），纯度≥95%，并提供质检报告。
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Q3-能否用于禽流感监测？
可以，作为ELISA抗原检测禽类血清中的H11N2特异性抗体。
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Q4-是否提供预包被ELISA板？
支持，提供预包被HA的96孔板，优化封闭条件，直接用于抗体效价检测。
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Q5-运输和储存条件是什么？
液体需-80℃保存，冻干粉可常温运输，复溶后分装避免反复冻融。
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400-621-6806 -
614353794 -
周一至周五 9:00-18:00 -
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