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DENV Envelope Protein, His Tag

DENV Envelope Protein, His Tag

产品编号

KMP4429

别名

登革热病毒包膜蛋白, DENV Envelope, E Protein, DENV Envelope Protein

规格
  • 50ug
  • 100ug
  • 200ug
产品介绍
Alias登革热病毒包膜蛋白, DENV Envelope, E Protein, DENV Envelope Protein
Catalog NumberKMP4429
Product DescriptionThe DENV Envelope Protein(KMP4429) is produced in HEK293 Cells. A DNA sequence encoding the Dengue virus (DENV)(type 1, strain US/Hawaii/1944) E (ACF49259.1) (Val580-Lys680) was expressed with a polyhistidine tag at the C-terminus.
Molecular NameDENV Envelope
SpeciesDENV
HostHEK293 Cells
Size50ug, 100ug, 200ug
Purity>75% as determined by SDS-PAGE
PurificationAffinity purification
Endotoxin<1.0 EU/ug determined by the LAL method
Formulation250mM NaCl, 50mM Tris, pH8.0
BackgroundEnvelope of Zika virus is responsible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent with those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross-reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross-reactivity with dengue and YF. Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.
Storage ConditionAliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping ConditionIn general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

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