DENV (type 1, strain US/Hawaii/1944) Envelope Protein, His Tag
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产品编号
KMP4428
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别名
登革热病毒包膜蛋白, DENV Envelope, E Protein, DENV Envelope Protein
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规格
- 50ug
- 100ug
- 200ug
产品介绍
| Alias | 登革热病毒包膜蛋白, DENV Envelope, E Protein, DENV Envelope Protein |
| Catalog Number | KMP4428 |
| Product Description | The DENV (type 1, strain US/Hawaii/1944) Envelope Protein(KMP4428) is produced in Insect Cells. A DNA sequence encoding the Dengue virus (DENV)(type 1, strain US/Hawaii/1944) E (ACF49259.1) (Val580-Lys680) was expressed with a polyhistidine tag at the C-terminus. |
| Molecular Name | DENV Envelope |
| Species | DENV |
| Host | Insect Cells |
| Size | 50ug, 100ug, 200ug |
| Purity | >95% as determined by SDS-PAGE |
| Purification | Affinity purification |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Formulation | PBS, pH7.4 |
| Background | Envelope of Zika virus is responsible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent with those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross-reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross-reactivity with dengue and YF. Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus. |
| Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
FAQ
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Q1-该包膜蛋白是否保留血凝活性？
是的，通过血凝试验（HAU）验证，每μg蛋白可凝集≥1×10^6鹅红细胞。
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Q2-能否用于登革热早期诊断？
是的，作为ELISA抗原检测患者血清中的IgM抗体，区分急性感染。
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Q3-如何控制内毒素水平？
采用无内毒素表达系统（如HEK293），并通过LAL检测确保内毒素＜0.1 EU/μg。
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Q4-是否支持动物免疫实验？
支持，配合铝佐剂或TLR激动剂，诱导高滴度中和抗体。
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Q5-运输条件有何要求？
液体需干冰运输，冻干粉可常温运输，附带温度记录仪。
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400-621-6806 -
614353794 -
周一至周五 9:00-18:00 -
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