DENV (type 2, strain New Guinea C/PUO-218 hybrid) Envelope Protein, His Tag
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产品编号
KMP4424
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别名
登革热病毒包膜蛋白, DENV Envelope, E Protein, DENV Envelope Protein
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规格
- 50ug
- 100ug
- 200ug
产品介绍
| Alias | 登革热病毒包膜蛋白, DENV Envelope, E Protein, DENV Envelope Protein |
| Catalog Number | KMP4424 |
| Product Description | The DENV (type 2, strain New Guinea C/PUO-218 hybrid) Envelope Protein(KMP4424) is produced in Insect Cells. A DNA sequence encoding the Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E (AAC59274.1, with mutation Thr 76 Arg, Gln 77 Glu, Trp 101 Arg, Leu 107 Arg) (Met281-Gly679) was expressed with a polyhistidine tag at the C-terminus. |
| Molecular Name | DENV Envelope |
| Species | DENV |
| Host | Insect Cells |
| Size | 50ug, 100ug, 200ug |
| Purity | >90% as determined by SDS-PAGE |
| Purification | Affinity purification |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Formulation | 20mM Tris, 500mM NaCl, pH8.0 |
| Background | Envelope of Zika virus is responsible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent with those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross-reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross-reactivity with dengue and YF. Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus. |
| Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
FAQ
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Q1-该包膜蛋白是否覆盖不同登革毒株的表位？
是的，为嵌合蛋白设计，涵盖PUO-218株的关键中和表位，增强抗体检测广谱性。
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Q2-能否用于四价登革疫苗研发？
支持，作为血清型2的候选抗原，与其他血清型包膜蛋白组合使用。
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Q3-如何验证蛋白的天然构象？
通过ELISA检测其与型特异性单克隆抗体（如3H5）的结合能力。
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Q4-是否支持假病毒包装？
可以，与DENV其他结构蛋白共表达，构建假病毒用于中和实验。
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Q5-运输和储存条件是什么？
液体需-80℃保存，冻干粉可常温运输，复溶后避免反复冻融。
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400-621-6806 -
614353794 -
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