DENV (type 2, strain New Guinea C) NS1 Protein, His Tag
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产品编号
KMP4422
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别名
登革热病毒糖蛋白NS1, DENV NS1, Nonstructural Protein 1, DENV NS1 Protein
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规格
- 50ug
- 100ug
- 200ug
产品介绍
| Alias | 登革热病毒糖蛋白NS1, DENV NS1, Nonstructural Protein 1, DENV NS1 Protein |
| Catalog Number | KMP4422 |
| Product Description | The DENV (type 2, strain New Guinea C) NS1 Protein(KMP4422) is produced in HEK293 Cells. A DNA sequence encoding the Dengue virus (type 2, strain New Guinea C) NS1 (AAC59275.1) (Asp1-Ala352) was expressed with a polyhistidine tag at the N-terminus. |
| Molecular Name | DENV NS1 |
| Species | DENV |
| Host | HEK293 Cells |
| Size | 50ug, 100ug, 200ug |
| Purity | >95% as determined by SDS-PAGE |
| Purification | Affinity purification |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Formulation | PBS, pH7.4 |
| Background | Envelope of Zika virus is responsible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent with those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross-reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross-reactivity with dengue and YF. Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus. |
| Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
FAQ
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Q1-NS1蛋白在登革热诊断中的价值是什么？
NS1是早期感染标志物，用于ELISA或快速试纸条检测急性期患者血清中的抗原。
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Q2-如何避免与其他黄病毒（如寨卡病毒）的交叉反应？
采用表位特异性纯化和交叉吸附技术，确保检测特异性＞95%。
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Q3-能否用于疫苗研发？
支持，作为亚单位疫苗抗原，已在小鼠模型中诱导保护性免疫应答。
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Q4-储存时是否需要添加稳定剂？
建议添加1% BSA和0.01% Tween-20，防止冻融过程中蛋白聚集。
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Q5-是否提供预包被ELISA板？
支持，提供预包被NS1的96孔板，优化封闭条件，直接用于检测。
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