CV (strain G-10) VP1 Protein, Fc Tag
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产品编号
KMP4418
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别名
柯萨奇病毒衣壳蛋白VP1, CV VP1, Capsid Protein VP1, CV VP1 Protein
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规格
- 50ug
- 100ug
- 200ug
产品介绍
| Alias | 柯萨奇病毒衣壳蛋白VP1, CV VP1, Capsid Protein VP1, CV VP1 Protein |
| Catalog Number | KMP4418 |
| Product Description | The CV (strain G-10) VP1 Protein(KMP4418) is produced in Insect Cells. A DNA sequence encoding the VP1 of Coxsackievirus A16 (Cox A16) (strain G-10) (AAA50478.1) (Gly568-Lys862) was expressed with the Fc region of human IgG1 at the C-terminus. |
| Molecular Name | CV VP1 |
| Species | CV |
| Host | Insect Cells |
| Size | 50ug, 100ug, 200ug |
| Purity | >90% as determined by SDS-PAGE |
| Purification | Affinity purification |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Formulation | 40mM PB, 500mM NaCl, pH7.4 |
| Background | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
FAQ
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Q1-VP1蛋白在柯萨奇病毒中的功能是什么？
VP1是病毒衣壳主要结构蛋白，参与宿主细胞受体结合，是中和抗体检测的核心抗原。
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Q2-Fc标签的作用是什么？
Fc标签延长蛋白半衰期，促进抗原呈递，增强疫苗的体液免疫应答。
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Q3-能否用于手足口病诊断试剂开发？
是的，作为ELISA或免疫层析试纸条的捕获抗原，区分CV与其他肠道病毒感染。
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Q4-蛋白纯度如何控制？
通过亲和层析和分子筛色谱纯化，纯度≥95%，并提供SDS-PAGE和HPLC报告。
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Q5-是否支持定制其他柯萨奇病毒抗原？
支持，可定制CV A6、A10等血清型的VP1或VP2蛋白。
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