CHIKV Envelope 2 Protein, His Tag
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产品编号
KMP4417
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别名
基孔肯雅热病毒包膜蛋白2, CHIKV Envelope 2, E2 Protein, CHIKV Envelope 2 Protein
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规格
- 50ug
- 100ug
- 200ug
产品介绍
| Alias | 基孔肯雅热病毒包膜蛋白2, CHIKV Envelope 2, E2 Protein, CHIKV Envelope 2 Protein |
| Catalog Number | KMP4417 |
| Product Description | The CHIKV Envelope 2 Protein(KMP4417) is produced in Insect Cells. A DNA sequence encoding the Chikungunya virus (CHIKV)(strain SL-CK1) E2/Envelope 2 (ADG95913.1) (Ser326-Gln666) was expressed with a polyhistidine tag at the C-terminus. |
| Molecular Name | CHIKV Envelope 2 |
| Species | CHIKV |
| Host | Insect Cells |
| Size | 50ug, 100ug, 200ug |
| Purity | >95% as determined by SDS-PAGE |
| Purification | Affinity purification |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Formulation | 40mM PB, 500mM NaCl, pH7.4 |
| Background | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
FAQ
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Q1-Envelope 2蛋白在基孔肯雅病毒中的功能是什么？
Envelope 2（E2）是病毒表面糖蛋白，介导宿主细胞受体结合和膜融合，是疫苗和中和抗体的关键靶点。
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Q2-能否用于快速诊断试纸条开发？
是的，作为胶体金或荧光标记抗原，检测患者血清中的IgM/IgG抗体。
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Q3-如何避免与登革热病毒的交叉反应？
通过表位特异性纯化和交叉吸附技术，确保抗原特异性＞98%。
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Q4-是否支持动物免疫实验？
支持，提供铝佐剂或Poly IC配伍方案，已用于小鼠模型诱导中和抗体。
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Q5-储存条件有何要求？
建议-80℃保存，避免反复冻融，短期使用可暂存于-20℃。
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