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Rabbit anti Human Perilipin-2(PLIN2) Monoclonal Antibody

Rabbit anti Human Perilipin-2(PLIN2) Monoclonal Antibody

产品编号

PA17214

别名

ADFP antibody, ADRP antibody, Perilipin-2 antibody, PLIN2 HUMAN, Perilipin-2, ADFP, Adipophilin, Adipose differentiation-related protein

规格
  • 50ul
  • 100ul
  • 200ul
产品介绍
AliasADFP antibody, ADRP antibody, Perilipin-2 antibody, PLIN2 HUMAN, Perilipin-2, ADFP, Adipophilin, Adipose differentiation-related protein
Catalog NumberPA17214
Product DescriptionThe Rabbit Anti Human PLIN2 monoclonal Antibody was obtained by affinity purification from a rabbit immunized with a purified synthetic peptide corresponding to a sequence within amino acids 35-134 of human BNP (NP_002512.1)
HostRabbit
Size50ul, 100ul, 200ul
ClonityMonoclonal
PurificationAffinity purification
FormulationPBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
BackgroundThe protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 88-437 of human Perilipin-2 (NP_001113.2)
Storage ConditionStore at -20℃. Avoid freeze / thaw cycles.
Shipping ConditionThis antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stabilitystable at -20 ° C
Cross-reactivityHuman, Mouse, Rat
Product Declaration本产品仅用于研究用途(RUO)。不能用于诊断或直接用于人体实验。
FAQ
  • Q1- 是否用于脂肪肝或肥胖研究?

    是,检测肝细胞或脂肪细胞中PLIN2包被的脂滴,IHC(1:200)或IF(1:500)显示脂滴分布。

  • Q2- 是否区分PLIN2与其他Perilipin家族成员(如PLIN1或PLIN3)?

    特异性结合PLIN2,交叉反应率<0.1%(经PLIN1/PLIN3 KO细胞验证)。

  • Q3- 如何处理富含脂质的样本?

    组织切片需OCT包埋快速冷冻,避免脂质氧化;WB裂解液含1% Triton X-100溶解脂滴。

  • Q4- 是否支持流式细胞术?

    需透膜处理(0.1%皂苷),推荐1:50稀释检测胞内PLIN2。

  • Q5- 保存条件?

    冻干粉-80℃保存5年,复溶后4℃保存1周(含0.1% BSA)。

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